Composite

Part:BBa_K4060523:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BBa_K4060513 + BBa_K4060517


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3064
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal NheI site found at 2551
    Illegal NheI site found at 2574
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3064
    Illegal BamHI site found at 619
    Illegal XhoI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 1853
    Illegal NgoMIV site found at 2355
    Illegal NgoMIV site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1410
    Illegal BsaI.rc site found at 2256


Design Notes

The construction of this part is according to igem assembly protocol


Source

The BphP1 gene and QPAS1 gene are cloned from Addgene plasmid pKA-207I10. The LexA gene is cloned from E.coli K12 MG1655 genomic DNA. The remaining parts are from 2019 igem kits.

References